trpv1 cre mice Search Results


mouse b6 129 trpv1 tm1 cre bbm j  (Jackson Laboratory)
86
Jackson Laboratory mouse b6 129 trpv1 tm1 cre bbm j
Schistosoma mansoni infection reduces <t>TRPV1+</t> neuron responses. (A) Experimental approach for percutaneous exposure to S. mansoni in the paw followed by assessment of thermal pain sensitivity 1 d after infection and DRG collection 2 d post-infection. DRG cultures were subsequently evaluated for capsaicin-induced calcium influx and neuropeptide release. (B) Thermal pain sensitivity in the ipsilateral (exposed) and contralateral (unexposed) paws of naïve and S. mansoni infected mice assessed by withdrawal times (seconds) to infrared heat. (C, D) Representative image of calcium traces of DRG neuron cultures generated from wild-type naïve or 2-d S. mansoni -infected mice. DRG neurons were sequentially treated with 1 μM capsaicin for 5 minutes followed by stimulation with 70 mM KCl for 1 minute. (E), Percentage of capsaicin-responsive DRG neurons per coverslip in naïve and S. mansoni -infected mice. (F) Area under the curve (AUC) values of intracellular calcium levels of wildtype DRG neurons from naïve or S. mansoni -infected mice during capsaicin treatment. (G, H), CGRP and Substance P supernatant levels of wildtype DRG neurons generated from wildtype naïve, or 2-d S. mansoni -infected ipsilateral and contralateral paws treated with vehicle or 1 μM capsaicin for 1 h. P values were determined by 2-tailed Student t -tests, or One-way ANOVA with post hoc correction. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2-3 independent experiments, each with ≥4 biological replicates. Panel A was created in Biorender.
Mouse B6 129 Trpv1 Tm1 Cre Bbm J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trpv1+cre+mice/pmc12576132-71-0-6?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
mouse b6 129 trpv1 tm1 cre bbm j - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier

Image Search Results


Schistosoma mansoni infection reduces TRPV1+ neuron responses. (A) Experimental approach for percutaneous exposure to S. mansoni in the paw followed by assessment of thermal pain sensitivity 1 d after infection and DRG collection 2 d post-infection. DRG cultures were subsequently evaluated for capsaicin-induced calcium influx and neuropeptide release. (B) Thermal pain sensitivity in the ipsilateral (exposed) and contralateral (unexposed) paws of naïve and S. mansoni infected mice assessed by withdrawal times (seconds) to infrared heat. (C, D) Representative image of calcium traces of DRG neuron cultures generated from wild-type naïve or 2-d S. mansoni -infected mice. DRG neurons were sequentially treated with 1 μM capsaicin for 5 minutes followed by stimulation with 70 mM KCl for 1 minute. (E), Percentage of capsaicin-responsive DRG neurons per coverslip in naïve and S. mansoni -infected mice. (F) Area under the curve (AUC) values of intracellular calcium levels of wildtype DRG neurons from naïve or S. mansoni -infected mice during capsaicin treatment. (G, H), CGRP and Substance P supernatant levels of wildtype DRG neurons generated from wildtype naïve, or 2-d S. mansoni -infected ipsilateral and contralateral paws treated with vehicle or 1 μM capsaicin for 1 h. P values were determined by 2-tailed Student t -tests, or One-way ANOVA with post hoc correction. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2-3 independent experiments, each with ≥4 biological replicates. Panel A was created in Biorender.

Journal: The Journal of Immunology Author Choice

Article Title: TRPV1+ neurons promote cutaneous immunity against Schistosoma mansoni

doi: 10.1093/jimmun/vkaf141

Figure Lengend Snippet: Schistosoma mansoni infection reduces TRPV1+ neuron responses. (A) Experimental approach for percutaneous exposure to S. mansoni in the paw followed by assessment of thermal pain sensitivity 1 d after infection and DRG collection 2 d post-infection. DRG cultures were subsequently evaluated for capsaicin-induced calcium influx and neuropeptide release. (B) Thermal pain sensitivity in the ipsilateral (exposed) and contralateral (unexposed) paws of naïve and S. mansoni infected mice assessed by withdrawal times (seconds) to infrared heat. (C, D) Representative image of calcium traces of DRG neuron cultures generated from wild-type naïve or 2-d S. mansoni -infected mice. DRG neurons were sequentially treated with 1 μM capsaicin for 5 minutes followed by stimulation with 70 mM KCl for 1 minute. (E), Percentage of capsaicin-responsive DRG neurons per coverslip in naïve and S. mansoni -infected mice. (F) Area under the curve (AUC) values of intracellular calcium levels of wildtype DRG neurons from naïve or S. mansoni -infected mice during capsaicin treatment. (G, H), CGRP and Substance P supernatant levels of wildtype DRG neurons generated from wildtype naïve, or 2-d S. mansoni -infected ipsilateral and contralateral paws treated with vehicle or 1 μM capsaicin for 1 h. P values were determined by 2-tailed Student t -tests, or One-way ANOVA with post hoc correction. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2-3 independent experiments, each with ≥4 biological replicates. Panel A was created in Biorender.

Article Snippet: Mouse: B6.129- Trpv1 tm1(cre)Bbm /J , The Jackson Laboratory , Strain #017769.

Techniques: Infection, Generated

TRPV1+ neuron activation confers resistance to S. mansoni and induces cytokine expression in γδ T cells. (A) Experimental approach for optogenetic ear stimulation of ChR2 control or TRPV1-ChR2 mice followed by evaluation of skin resistance after exposure to 150–200 S. mansoni cercariae for 30 min or lung larval (schistosomulae) load 6 d post-infection with 500 S. mansoni cercariae. Life cycle is depicted for reference. (B) Ear thickness measured daily during photostimulation of ChR2 control or TRPV1-ChR2 mice. (C) Percentage of non-penetrating cercariae from ChR2 control or TRPV1-ChR2 mice that were previously photostimulated as shown in panel A. (D) Lung schistosomulae of light-stimulated ChR2 control or TRPV1-ChR2 mice quantified 6 d post-infection. (E) Percentage of IL-17+ cells in the skin of control or TRPV1-ChR2 mice after 5 d of blue light stimulation followed by S. mansoni infection. (F), Pie charts depicting the percentage of lymphocyte cellular subsets within IL-17+ cells in the skin of mice from E. (G, H), Representative dot plots and quantification of IL-17+ dermal γδ T cells (CD45+ CD90+ TCRδ+) and (I, J), CD4+ Th cells (CD45+ CD90+ TCRβ+CD4+Foxp3-) by flow cytometry of control or TRPV1-ChR2 mice optogenetically stimulated for 5 d followed by S. mansoni infection for 1 d. (K, L) Representative dot plots, percentages, and absolute numbers of Ki-67+ and (M, N) IL-13+ γδ T cells quantified by flow cytometry. P values were determined by 2-tailed Student t -tests, or 2-way ANOVA with post hoc correction. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2-3 independent experiments, each with ≥4 biological replicates. Panel A was created in Biorender.

Journal: The Journal of Immunology Author Choice

Article Title: TRPV1+ neurons promote cutaneous immunity against Schistosoma mansoni

doi: 10.1093/jimmun/vkaf141

Figure Lengend Snippet: TRPV1+ neuron activation confers resistance to S. mansoni and induces cytokine expression in γδ T cells. (A) Experimental approach for optogenetic ear stimulation of ChR2 control or TRPV1-ChR2 mice followed by evaluation of skin resistance after exposure to 150–200 S. mansoni cercariae for 30 min or lung larval (schistosomulae) load 6 d post-infection with 500 S. mansoni cercariae. Life cycle is depicted for reference. (B) Ear thickness measured daily during photostimulation of ChR2 control or TRPV1-ChR2 mice. (C) Percentage of non-penetrating cercariae from ChR2 control or TRPV1-ChR2 mice that were previously photostimulated as shown in panel A. (D) Lung schistosomulae of light-stimulated ChR2 control or TRPV1-ChR2 mice quantified 6 d post-infection. (E) Percentage of IL-17+ cells in the skin of control or TRPV1-ChR2 mice after 5 d of blue light stimulation followed by S. mansoni infection. (F), Pie charts depicting the percentage of lymphocyte cellular subsets within IL-17+ cells in the skin of mice from E. (G, H), Representative dot plots and quantification of IL-17+ dermal γδ T cells (CD45+ CD90+ TCRδ+) and (I, J), CD4+ Th cells (CD45+ CD90+ TCRβ+CD4+Foxp3-) by flow cytometry of control or TRPV1-ChR2 mice optogenetically stimulated for 5 d followed by S. mansoni infection for 1 d. (K, L) Representative dot plots, percentages, and absolute numbers of Ki-67+ and (M, N) IL-13+ γδ T cells quantified by flow cytometry. P values were determined by 2-tailed Student t -tests, or 2-way ANOVA with post hoc correction. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2-3 independent experiments, each with ≥4 biological replicates. Panel A was created in Biorender.

Article Snippet: Mouse: B6.129- Trpv1 tm1(cre)Bbm /J , The Jackson Laboratory , Strain #017769.

Techniques: Activation Assay, Expressing, Control, Infection, Flow Cytometry

TRPV1+ neurons promote neutrophil and monocyte recruitment and activation after S. mansoni infection. (A) Ear thickness of light-stimulated control or TRPV1-ChR2 mice measured 1 d after exposure to S. mansoni cercariae. (B), Representative dot plots of neutrophils (CD11b+ Ly6G+ Ly6Cint) and monocytes (CD11b+ Ly6G- Ly6Chi) assessed by flow cytometry of light-stimulated control or TRPV1-ChR2 mice 1 d after S. mansoni infection. (C, D) Percentage and absolute numbers of neutrophils and monocytes from S. mansoni -infected control or TRPV1-ChR2 mice. (E–G) Representative dot plots and quantification of iNOS expression in neutrophils and monocytes assessed by flow cytometry of light-stimulated control or TRPV1-ChR2 mice 1 d after S. mansoni infection. (H) Representative histogram and gMFI quantification of iNOS expression in neutrophils and (I), monocytes in the skin of light-stimulated control or TRPV1-ChR2 mice 1 d after S. mansoni infection. P values were determined by two-tailed Student’s t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2–3 independent experiments, each with ≥4 biological replicates.

Journal: The Journal of Immunology Author Choice

Article Title: TRPV1+ neurons promote cutaneous immunity against Schistosoma mansoni

doi: 10.1093/jimmun/vkaf141

Figure Lengend Snippet: TRPV1+ neurons promote neutrophil and monocyte recruitment and activation after S. mansoni infection. (A) Ear thickness of light-stimulated control or TRPV1-ChR2 mice measured 1 d after exposure to S. mansoni cercariae. (B), Representative dot plots of neutrophils (CD11b+ Ly6G+ Ly6Cint) and monocytes (CD11b+ Ly6G- Ly6Chi) assessed by flow cytometry of light-stimulated control or TRPV1-ChR2 mice 1 d after S. mansoni infection. (C, D) Percentage and absolute numbers of neutrophils and monocytes from S. mansoni -infected control or TRPV1-ChR2 mice. (E–G) Representative dot plots and quantification of iNOS expression in neutrophils and monocytes assessed by flow cytometry of light-stimulated control or TRPV1-ChR2 mice 1 d after S. mansoni infection. (H) Representative histogram and gMFI quantification of iNOS expression in neutrophils and (I), monocytes in the skin of light-stimulated control or TRPV1-ChR2 mice 1 d after S. mansoni infection. P values were determined by two-tailed Student’s t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2–3 independent experiments, each with ≥4 biological replicates.

Article Snippet: Mouse: B6.129- Trpv1 tm1(cre)Bbm /J , The Jackson Laboratory , Strain #017769.

Techniques: Activation Assay, Infection, Control, Flow Cytometry, Expressing, Two Tailed Test

TRPV1+ neurons are required to mitigate S. mansoni lung larval burden, γδ T cell responses and myeloid cell skin accumulation. (A) Thermal pain sensitivity in the paws of wildtype mice treated with vehicle or resiniferatoxin (RTX) assessed by withdrawal times (seconds) to infrared heat. (B, C) Percentage of non-penetrating cercariae and d6 lung larval burden from vehicle or RTX-treated mice infected with S. mansoni. (D) Percentage and absolute numbers of IL-17+, (E) Ki67+, and (F), IL-13+ γδ T cells 1 day post-infection with S. mansoni in vehicle or RTX-treated mice. (G, H) Skin neutrophils and monocytes from vehicle- or RTX-treated mice infected with S. mansoni . P values were determined by two-tailed Student’s t-test. * P < 0.0 5, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2–3 independent experiments, each with ≥5 biological replicates.

Journal: The Journal of Immunology Author Choice

Article Title: TRPV1+ neurons promote cutaneous immunity against Schistosoma mansoni

doi: 10.1093/jimmun/vkaf141

Figure Lengend Snippet: TRPV1+ neurons are required to mitigate S. mansoni lung larval burden, γδ T cell responses and myeloid cell skin accumulation. (A) Thermal pain sensitivity in the paws of wildtype mice treated with vehicle or resiniferatoxin (RTX) assessed by withdrawal times (seconds) to infrared heat. (B, C) Percentage of non-penetrating cercariae and d6 lung larval burden from vehicle or RTX-treated mice infected with S. mansoni. (D) Percentage and absolute numbers of IL-17+, (E) Ki67+, and (F), IL-13+ γδ T cells 1 day post-infection with S. mansoni in vehicle or RTX-treated mice. (G, H) Skin neutrophils and monocytes from vehicle- or RTX-treated mice infected with S. mansoni . P values were determined by two-tailed Student’s t-test. * P < 0.0 5, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Representative of 2–3 independent experiments, each with ≥5 biological replicates.

Article Snippet: Mouse: B6.129- Trpv1 tm1(cre)Bbm /J , The Jackson Laboratory , Strain #017769.

Techniques: Infection, Two Tailed Test